Dynein polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Dynein.
Immunogen
A synthetic peptide corresponding to rat Dynein.
Host
Rabbit
Reactivity
Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of extract dynein from pig brain cytoplasmic (lane 1) , 14S dynein from Tetrahymena Axonemes (lane 2) and 22S dynein from Tetrahymena Axonemes (lane3) , using Dynein polyclonal antibody (Cat # PAB8509) . -
Publication Reference
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Regulation of monomeric dynein activity by ATP and ADP concentrations.
Shiroguchi K, Toyoshima YY.
Cell Motility and the Cytoskeleton 2001 Aug; 49(4):189.
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Processive movement of single 22S dynein molecules occurs only at low ATP concentrations.
Hirakawa E, Higuchi H, Toyoshima YY.
PNAS 2000 Mar; 97(6):2533.
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Kinesin and cytoplasmic dynein binding to brain microsomes.
Yu H, Toyoshima I, Steuer ER, Sheetz MP.
The Journal of Biological Chemistry 1992 Oct; 267(28):20457.
Application:Func, WB-Ce, WB-Ti, Chicken, Chicken embryonic brains.
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Cytoplasmic dynein is a vesicle protein.
Lacey ML, Haimo LT.
The Journal of Biological Chemistry 1992 Mar; 267(7):4793.
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Regulation of monomeric dynein activity by ATP and ADP concentrations.
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