MDC1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MDC1.
Immunogen
A synthetic peptide corresponding to human MDC1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of extract from 293T (lane 1) and HeLa cells (lane 2) , using MDC1 polyclonal antibody (Cat # PAB8487) . -
Gene Info — MDC1
Entrez GeneID
9656Gene Name
MDC1
Gene Alias
DKFZp781A0122, KIAA0170, MGC166888, NFBD1
Gene Description
mediator of DNA damage checkpoint 1
Omim ID
607593Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq
Other Designations
OTTHUMP00000029090|homologue to Drosophila photoreceptor protein calphotin|nuclear factor with BRCT domains 1
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Interactome
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Disease
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Publication Reference
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Mediator of DNA damage checkpoint protein 1 regulates BRCA1 localization and phosphorylation in DNA damage checkpoint control.
Lou Z, Chini CC, Minter-Dykhouse K, Chen J.
The Journal of Biological Chemistry 2003 Apr; 278(16):13599.
Application:IF, IP, WB, Human, HeLa cells.
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MDC1 is required for the intra-S-phase DNA damage checkpoint.
Goldberg M, Stucki M, Falck J, D'Amours D, Rahman D, Pappin D, Bartek J, Jackson SP.
Nature 2003 Feb; 421(6926):952.
Application:IF, IP, WB, Human, HeLa cells.
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MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.
Lou Z, Minter-Dykhouse K, Wu X, Chen J.
Nature 2003 Feb; 421(6926):957.
Application:IF, WB, Human, HEK 293T, K-562 cells.
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NFBD1, like 53BP1, is an early and redundant transducer mediating Chk2 phosphorylation in response to DNA damage.
Peng A, Chen PL.
The Journal of Biological Chemistry 2003 Mar; 278(11):8873.
Application:IS, WB-Tr, Human, MCF-7 cells.
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NFBD1/KIAA0170 is a chromatin-associated protein involved in DNA damage signaling pathways.
Xu X, Stern DF.
The Journal of Biological Chemistry 2002 Mar; 278(10):8795.
Application:IF, IP, IP-WB, WB-Ce, WB-Tr, Human, HEK 293, HeLa cells.
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Mediator of DNA damage checkpoint protein 1 regulates BRCA1 localization and phosphorylation in DNA damage checkpoint control.
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