GIT1 (phospho Y554) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of GIT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y554 of human GIT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of GIT1 (phospho Y554) polyclonal antibody (Cat # PAB8161) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — GIT1
Entrez GeneID
28964Protein Accession#
NP_054749;Q9Y2X7Gene Name
GIT1
Gene Alias
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Gene Description
G protein-coupled receptor kinase interacting ArfGAP 1
Omim ID
608434Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor kinase interactor 1|G protein-coupled receptor kinase-interactor 1|OTTHUMP00000163606
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of adaptor protein GIT1 in platelets, leading to the interaction between GIT1 and integrin alpha(IIb)beta3.
Sato H, Suzuki-Inoue K, Inoue O, Ozaki Y.
Biochemical and Biophysical Research Communications 2008 Jan; 368(1):157.
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Role of phospholipase Cgamma1 in cell spreading requires association with a beta-Pix/GIT1-containing complex, leading to activation of Cdc42 and Rac1.
Jones NP, Katan M.
Molecular and Cellular Biology 2007 Aug; 27(16):5790.
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GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin.
Schmalzigaug R, Garron ML, Roseman JT, Xing Y, Davidson CE, Arold ST, Premont RT.
Cellular Signalling 2007 Aug; 19(8):1733.
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Regulation of adaptor protein GIT1 in platelets, leading to the interaction between GIT1 and integrin alpha(IIb)beta3.
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