PDPK1 (phospho S396) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S396 of human PDPK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of PDPK1 (phospho S396) polyclonal antibody (Cat # PAB8131) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — PDPK1
Entrez GeneID
5170Protein Accession#
NP_002604;O15530Gene Name
PDPK1
Gene Alias
MGC20087, MGC35290, PDK1, PRO0461
Gene Description
3-phosphoinositide dependent protein kinase-1
Omim ID
605213Gene Ontology
HyperlinkOther Designations
PkB kinase like gene 1|PkB-like 1|protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
Nilsen T, Slagsvold T, Skjerpen CS, Brech A, Stenmark H, Olsnes S.
The Journal of Biological Chemistry 2003 Nov; 279(6):4794.
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In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.
Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR.
The EMBO Journal 2003 Aug; 22(16):4202.
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Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A.
The Journal of Biological Chemistry 2002 Jul; 277(41):38863.
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Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
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