Fas polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Fas.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of rat Fas.
Host
Rabbit
Theoretical MW (kDa)
55, 37.7
Reactivity
Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunohistochemistry (Frozen sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — Fas
Entrez GeneID
246097Gene Name
Fas
Gene Alias
Tnfrsf6
Gene Description
Fas (TNF receptor superfamily, member 6)
Gene Ontology
HyperlinkGene Summary
I
Other Designations
CD95 antigen|Fas antigen (ATP1)|Fas receptor|Tumor necrosis factor receptor superfamily, member 6|apo-1 antigen|apoptosis-mediating surface antigen FAS|tumor necrosis factor receptor superfamily, member 6
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Publication Reference
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Quercetin inhibition of SREBPs and ChREBP expression results in reduced cholesterol and fatty acid synthesis in C6 glioma cells.
Damiano F, Giannotti L, Gnoni GV, Siculella L, Gnoni A.
The International Journal of Biochemistry & Cell Biology 2019 Sep; 117:105618.
Application：WB-Ce, Rat, C6 cells.
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Wogonoside induces depalmitoylation and translocation of PLSCR1 and N-RAS in primary acute myeloid leukaemia cells.
Li H, Yu X, Liu X, Hu P, Shen L, Zhou Y, Zhu Y, Li Z, Hui H, Guo Q, Xu J.
Journal of Cellular and Molecular Medicine 2018 Apr; 22(4):2117.
Application：IF, WB, Human, Human primary acute myeloid leukaemia cells.
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Functional polymorphisms in cell death pathway genes FAS and FASL contribute to risk of lung cancer.
Zhang X, Miao X, Sun T, Tan W, Qu S, Xiong P, Zhou Y, Lin D.
Journal of Medical Genetics 2005 Jun; 42(6):479.
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Fas engagement induces neurite growth through ERK activation and p35 upregulation.
Desbarats J, Birge RB, Mimouni-Rongy M, Weinstein DE, Palerme JS, Newell MK.
Nature Cell Biology 2003 Feb; 5(2):118.
Application：Flow Cyt, WB, Human, Jurkat, SH-SY5Y cells.
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Fas (CD95) expression is up-regulated on papillary thyroid carcinoma.
Arscott PL, Stokes T, Myc A, Giordano TJ, Thompson NW, Baker JR Jr.
The Journal of Clinical Endocrinology and Metabolism 1999 Nov; 84(11):4246.
Application：Flow Cyt, IHC-P, Human, Cancer thyrocytes, Thyroid carcinoma.
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Quercetin inhibition of SREBPs and ChREBP expression results in reduced cholesterol and fatty acid synthesis in C6 glioma cells.
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