SKP2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SKP2.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human SKP2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunocytochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
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Gene Info — SKP2
Entrez GeneID
6502Gene Name
SKP2
Gene Alias
FBL1, FBXL1, FLB1, MGC1366
Gene Description
S-phase kinase-associated protein 2 (p45)
Omim ID
601436Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
CDK2/cyclin A-associated protein p45|S-phase kinase-associated protein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Skp2 suppresses p53-dependent apoptosis by inhibiting p300.
Kitagawa M, Lee SH, McCormick F.
Molecular Cell 2008 Feb; 29(2):217.
Application:WB-Tr, Human, HCT-116, U-2 OS cells.
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Role of the F-box protein Skp2 in lymphomagenesis.
Latres E, Chiarle R, Schulman BA, Pavletich NP, Pellicer A, Inghirami G, Pagano M.
PNAS 2001 Feb; 98(5):2515.
Application:IP-WB, WB-Ce, WB-Ti, Mouse, Thymocytes, Lymphoma.
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Skp2 suppresses p53-dependent apoptosis by inhibiting p300.
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