MIF polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MIF.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human MIF.
Host
Rabbit
Theoretical MW (kDa)
12.4
Reactivity
Human, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Immunocytochemistry/Immunofluorescence (5 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell extracts using MIF polyclonal antibody (Cat # PAB7990).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat kidney sections, stain MIF in cytoplasm DAB chromogenic reaction.Immunocytochemistry
Immunofluorescence
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Gene Info — MIF
Entrez GeneID
4282Gene Name
MIF
Gene Alias
GIF, GLIF, MMIF
Gene Description
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
Other Designations
glycosylation-inhibiting factor|phenylpyruvate tautomerase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment.
Bernhagen J, Krohn R, Lue H, Gregory JL, Zernecke A, Koenen RR, Dewor M, Georgiev I, Schober A, Leng L, Kooistra T, Fingerle-Rowson G, Ghezzi P, Kleemann R, McColl SR, Bucala R, Hickey MJ, Weber C.
Nature Medicine 2007 May; 13(5):587.
Application:Func, Human, Mouse, HAoECs, Human, mouse monocytes, MonoMac6 cells.
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Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2.
Budarf M, McDonald T, Sellinger B, Kozak C, Graham C, Wistow G.
Genomics 1997 Jan; 39(2):235.
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Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes.
Bozza M, Kolakowski LF Jr, Jenkins NA, Gilbert DJ, Copeland NG, David JR, Gerard C.
Genomics 1995 Jun; 27(3):412.
Application:WB-Ce, WB-Tr, Mouse, Mammalian cells.
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MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment.
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