CALB2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CALB2.
Immunogen
A synthetic peptide corresponding to amino acids 4-18 of human CALB2.
Sequence
PQQQPPYLHLAELTA
Host
Rabbit
Theoretical MW (kDa)
31.5
Reactivity
Human
Form
Lyophilized
Purification
Immunogen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide).
Storage Instruction
Store at -20°C.
After reconstitution with 0.2 mL of distilled water will yield a concentration of 500 ug/mL, store at 4°C for one month. Form long term storage, it can also be aliquoted and stored frozen at -20°C. Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate. Using CALB2 polyclonal antibody (Cat # PAB7957).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectal cancer. -
Gene Info — CALB2
Entrez GeneID
794Protein Accession#
P22676Gene Name
CALB2
Gene Alias
CAL2
Gene Description
calbindin 2
Omim ID
114051Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq
Other Designations
calbindin 2, (29kD, calretinin)|calbindin D29K|calretinin
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Interactome
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Disease
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Publication Reference
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The mouse calretinin gene promoter region: structural and functional components.
Strauss KI, Kuznicki J, Winsky L, Kawagoe JI, Hammer M, Jacobowitz DM.
Brain Research. Molecular Brain Research 1997 Oct; 49(1-2):175.
Application:IF, Mouse, Brain.
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Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.
Gabrielides C, McCormack AL, Hunt DF, Christakos S.
Biochemistry 1991 Jan; 30(3):656.
Application:WB-Ti, Chicken, Human, Rat, Chicken cerebellum, Human cerebellum, Rat cerebellum, Rat kidney.
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The mouse calretinin gene promoter region: structural and functional components.
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