API5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of API5.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human API5.
Host
Rabbit
Reactivity
Human, Mouse, Rabbit, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat cardiac muscle tissue lysate. Using API5 polyclonal antibody (Cat # PAB7951) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human epidermic carcinoma sections using API5 polyclonal antibody (Cat # PAB7951) . -
Gene Info — API5
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Interactome
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Publication Reference
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AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal.
Tewari M, Yu M, Ross B, Dean C, Giordano A, Rubin R.
Cancer Research 1997 Sep; 57(18):4063.
Application:WB-Re, WB-Tr, Mouse, NIH/3T3 cells, Recombinant protein.
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AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal.
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