Bcar1 (phospho Y751) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Bcar1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y751 of rat Bcar1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-657 in mouse p130 Cas.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Bcar1
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Publication Reference
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Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration.
Shin NY, Dise RS, Schneider-Mergener J, Ritchie MD, Kilkenny DM, Hanks SK.
The Journal of Biological Chemistry 2004 Sep; 279(37):38331.
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Phosphorylation-dependent cleavage of p130cas in apoptotic rat-1 cells.
Hoon Kim D, Jeon Choi S, Kook S, Kim W, Keun Song W.
Biochemical and Biophysical Research Communications 2003 Jan; 300(1):141.
Application：WB-Ce, Rat, Rat-1 cells.
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Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions.
Pellicena P, Miller WT.
J Biol Chem 2001 Jun; 276(30):28190.
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Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration.
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