Pxn (phospho S83) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PC-12 cells untreated (lane 1) or treated with NGF (200 ng/ml) for 60 min (lane 2). The blots were probed with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932).
Immunofluorescence
Immunocytochemical labeling of Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) in rabbit spleen fibroblasts. The cells were labeled with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) (right) , then detected using appropriate secondary antibodies conjugated to Cy3.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Pxn.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S83 of mouse Pxn.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This sequence is highly conserved in rat paxillin and is also found in all isoforms (a, b, g) of paxillin.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:10)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PC-12 cells untreated (lane 1) or treated with NGF (200 ng/ml) for 60 min (lane 2). The blots were probed with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling of Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) in rabbit spleen fibroblasts. The cells were labeled with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) (right) , then detected using appropriate secondary antibodies conjugated to Cy3.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Pxn
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Publication Reference
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Defining blood-induced microglia functions in neurodegeneration through multiomic profiling.
Andrew S Mendiola, Zhaoqi Yan, Karuna Dixit, Jeffrey R Johnson, Mehdi Bouhaddou, Anke Meyer-Franke, Min-Gyoung Shin, Yu Yong, Ayushi Agrawal, Eilidh MacDonald, Gayathri Muthukumar, Clairice Pearce, Nikhita Arun, Belinda Cabriga, Rosa Meza-Acevedo, Maria Del Pilar S Alzamora, Scott S Zamvil, Alexander R Pico, Jae Kyu Ryu, Nevan J Krogan, Katerina Akassoglou.
Nature Immunology 2023 Jul; 24(7):1173.
Application：IF, Mouse, Mouse bone-marrow- derived macrophages (BMDMs).
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Paxillin serves as an ERK-regulated scaffold for coordinating FAK and Rac activation in epithelial morphogenesis.
Ishibe S, Joly D, Liu ZX, Cantley LG.
Molecular Cell 2004 Oct; 16(2):257.
Application：IF, IP, WB, Mouse, mIMCD-3 cells.
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Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells.
Huang C, Borchers CH, Schaller MD, Jacobson K.
The Journal of Cell Biology 2004 Feb; 164(4):593.
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Defining blood-induced microglia functions in neurodegeneration through multiomic profiling.
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