PLCG1 (phospho Y775) polyclonal antibody
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More Files
- More Functions
Immunoprecipitation
Western blot analysis of PLCG1 immunoprecipitates from human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). The blots were probed with PLCG1 (phospho Y775) polyclonal antibody (Cat # PAB7929).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PLCG1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y775 of human PLCG1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence has high homology to the conserved site in rat and mouse PLCg1, and has low homology to PLCg2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:100)
Immunoprecipitation (1-5 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunoprecipitation
Western blot analysis of PLCG1 immunoprecipitates from human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). The blots were probed with PLCG1 (phospho Y775) polyclonal antibody (Cat # PAB7929).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLCG1
Entrez GeneID
5335Gene Name
PLCG1
Gene Alias
PLC-II, PLC1, PLC148, PLCgamma1
Gene Description
phospholipase C, gamma 1
Omim ID
172420Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1|OTTHUMP00000031787|OTTHUMP00000178982|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol
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Interactome
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Pathway
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Disease
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Publication Reference
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A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling.
Serrano CJ, Graham L, DeBell K, Rawat R, Veri MC, Bonvini E, Rellahan BL, Reischl IG.
Journal of Immunology 2005 May; 174(10):6233.
Application：WB, Chicken, DT40 cells.
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A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility.
Chen P, Murphy-Ullrich JE, Wells A.
The Journal of Cell Biology 1996 Aug; 134(3):689.
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Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation.
Obermeier A, Tinhofer I, Grunicke HH, Ullrich A.
The EMBO Journal 1996 Jan; 15(1):73.
Application：IP, WB-Tr, Human, HEK 293 cells.
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A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling.
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