JUP (phospho Y550) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of JUP immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse JUP monoclonal antibody. The blots were probed with either JUP (phospho Y550) polyclonal antibody (Cat # PAB7924). The immunoprecipitated JUP was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies. In addition, the JUP (phospho Y550) polyclonal antibody (Cat # PAB7924) was used in the presence of phospho-JUP (Tyr-550) peptide (lane 3) or phospho-JUP (Tyr-644) peptide (lane 4).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUP.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y550 of human JUP.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence is highly conserved in rat and mouse g-Catenin.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of JUP immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse JUP monoclonal antibody. The blots were probed with either JUP (phospho Y550) polyclonal antibody (Cat # PAB7924). The immunoprecipitated JUP was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies. In addition, the JUP (phospho Y550) polyclonal antibody (Cat # PAB7924) was used in the presence of phospho-JUP (Tyr-550) peptide (lane 3) or phospho-JUP (Tyr-644) peptide (lane 4).Enzyme-linked Immunoabsorbent Assay
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Gene Info — JUP
Entrez GeneID
3728Gene Name
JUP
Gene Alias
ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
Gene Description
junction plakoglobin
Gene Ontology
HyperlinkGene Summary
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000164735|catenin (cadherin-associated protein), gamma (80kD)|catenin (cadherin-associated protein), gamma 80kDa|gamma-catenin
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Interactome
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Pathway
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Disease
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Publication Reference
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Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.
Miravet S, Piedra J, Castano J, Raurell I, Franci C, Dunach M, Garcia de Herreros A.
Molecular and Cellular Biology 2003 Oct; 23(20):7391.
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A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.
McCrea PD, Turck CW, Gumbiner B.
Science 1991 Nov; 254(5036):1356.
Application：WB-Ce, Dog, Frog, A6, MDCK cells.
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Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.
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