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Last updated: 2016/12/4
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NFKBIA (phospho Y42) polyclonal antibody

  • Catalog # : PAB7922
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y42 of human NFKBIA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This peptide sequence has low homology to other IkB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000)
    Immunoprecipitation (5-10 ul)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y42) polyclonal antibody (Cat # PAB7922). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-42) peptide (lane 2), phospho-NFKBIA (Tyr-305) peptide (lane 3), or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4792
  • Gene Name:
  • NFKBIA
  • Gene Alias:
  • IKBA,MAD-3,NFKBI
  • Gene Description:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • Gene Summary:
  • NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
  • Other Designations:
  • IkappaBalpha,nuclear factor of kappa light chain gene enhancer in B-cells
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