NFKBIA (phospho Y305) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y305) polyclonal antibody (Cat # PAB7919). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-305) peptide (lane 2), phospho-NFKBIA (Tyr-42) peptide (lane 3) or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y305 of human NFKBIA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence has low homology to other IkB proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y305) polyclonal antibody (Cat # PAB7919). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-305) peptide (lane 2), phospho-NFKBIA (Tyr-42) peptide (lane 3) or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKBIA
Entrez GeneID
4792Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
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Publication Reference
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Hepatitis C virus NS5A and subgenomic replicon activate NF-kappaB via tyrosine phosphorylation of IkappaBalpha and its degradation by calpain protease.
Waris G, Livolsi A, Imbert V, Peyron JF, Siddiqui A.
The Journal of Biological Chemistry 2003 Oct; 278(42):40778.
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Activation of nuclear factor kappaB and Bcl-x survival gene expression by nerve growth factor requires tyrosine phosphorylation of IkappaBalpha.
Bui NT, Livolsi A, Peyron JF, Prehn JH.
The Journal of Cell Biology 2001 Feb; 152(4):753.
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Inducible phosphorylation of I kappa B alpha is not sufficient for its dissociation from NF-kappa B and is inhibited by protease inhibitors.
Finco TS, Beg AA, Baldwin AS Jr.
PNAS 1994 Dec; 91(25):11884.
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Hepatitis C virus NS5A and subgenomic replicon activate NF-kappaB via tyrosine phosphorylation of IkappaBalpha and its degradation by calpain protease.
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