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Last updated: 2016/10/23
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WASF1 (phospho Y125) polyclonal antibody

  • Catalog # : PAB7913
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of WASF1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y125 of human WASF1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125).
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot of human SYF cSrc-transformed cells untreated (lane 1) or treated (lane 2) with pervanadate (1 mM; 30 min). The blots were probed with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB7913).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8936
  • Gene Name:
  • WASF1
  • Gene Alias:
  • FLJ31482,KIAA0269,SCAR1,WAVE,WAVE1
  • Gene Description:
  • WAS protein family, member 1
  • Gene Summary:
  • The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016990,Wiskott-Aldrich syndrome protein family member 1,homology of dictyostelium scar 1,verprolin homology domain-containing protein 1
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