PRKCA (phospho S657/Y658) polyclonal antibody
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More Files
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Immunoprecipitation
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PRKCA antibody. Control (lane 1) and alkaline phosphatase treated (lane 2) precipitates were probed with PRKCA (phospho S657/Y658) polyclonal antibody (Cat # PAB7910). The latter shows no detection of PRKCA after phosphatase treatment.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKCA.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S657/Y658 of human PRKCA.
Host
Rabbit
Reactivity
Human, Rabbit, Rat
Specificity
This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition,
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PRKCA antibody. Control (lane 1) and alkaline phosphatase treated (lane 2) precipitates were probed with PRKCA (phospho S657/Y658) polyclonal antibody (Cat # PAB7910). The latter shows no detection of PRKCA after phosphatase treatment.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCA
Entrez GeneID
5578Gene Name
PRKCA
Gene Alias
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA
Gene Description
protein kinase C, alpha
Omim ID
176960Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
Other Designations
aging-associated gene 6|protein kinase C alpha type
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Interactome
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Pathway
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Disease
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Publication Reference
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Kawakami Y, Kitaura J, Yao L, McHenry RW, Kawakami Y, Newton AC, Kang S, Kato RM, Leitges M, Rawlings DJ, Kawakami T.
PNAS 2003 Aug; 100(16):9470.
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The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nishizuka Y.
Nature 1988 Aug; 334(6184):661.
Application：IF, IHC, Rat, Rat cerebellar cortex.
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
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