WASF1 (phospho Y150) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of human K-562 cells treated with pervanadate (1 mM, 30 min) (lane 1) then treated with alkaline phosphatase (lane 2). The blots were probed with WASF1 (phospho Y150) polyclonal antibody (Cat # PAB7906).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of WASF1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y150 of human WASF1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of human K-562 cells treated with pervanadate (1 mM, 30 min) (lane 1) then treated with alkaline phosphatase (lane 2). The blots were probed with WASF1 (phospho Y150) polyclonal antibody (Cat # PAB7906).Enzyme-linked Immunoabsorbent Assay
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Gene Info — WASF1
Entrez GeneID
8936Gene Name
WASF1
Gene Alias
FLJ31482, KIAA0269, SCAR1, WAVE, WAVE1
Gene Description
WAS protein family, member 1
Omim ID
605035Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016990|Wiskott-Aldrich syndrome protein family member 1|homology of dictyostelium scar 1|verprolin homology domain-containing protein 1
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Interactome
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Pathway
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Publication Reference
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Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.
Leng Y, Zhang J, Badour K, Arpaia E, Freeman S, Cheung P, Siu M, Siminovitch K.
PNAS 2005 Jan; 102(4):1098.
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Crystal growth of carbonate apatite using a CaCO3 flux.
Suetsugu Y, Tanaka J.
Journal of Materials Science. Materials in Medicine 1999 Sep; 10(9):561.
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Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.
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