TUBB3 (phospho S172) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903).
Immunofluorescence
Immunocytochemical labeling in C2C12 cells using TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (A). The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of TUBB3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S172 of human TUBB3.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This sequence is identical to similar regions in bI, bII, and bIII-tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling in C2C12 cells using TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (A). The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (B).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBB3
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Interactome
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Pathway
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Publication Reference
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
Fourest-Lieuvin A, Peris L, Gache V, Garcia-Saez I, Juillan-Binard C, Lantez V, Job D.
Molecular Biology of the Cell 2005 Dec; 17(3):1041.
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Post-translational modifications regulate microtubule function.
Westermann S, Weber K.
Nature Reviews. Molecular Cell Biology 2003 Dec; 4(12):938.
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Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fanarraga ML, Avila J, Zabala JC.
The European Journal of Neuroscience 1999 Feb; 11(2):517.
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
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