CFL1 (phospho S3) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot of Jurkat cells expressing CFL1. Blots were treated with (lanes 2, 3) or without (lane 1) lambda phosphatase for 1 hr at 37 °C. In lane 3, phosphatase treatments were performed in the presence of phospho-CFL1 (Ser-3) peptide. Blots were probed with CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900) (lane 1). Phosphatase treatment removes Ser3 phosphorylation of CFL1, but this phosphatase activity is reduced in the presence of peptide.
Immunofluorescence
Immunocytochemical labeling in chick dorsal root ganglion neurons using CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900). (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CFL1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to resdiues surrounding S3 of human CFL1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of Jurkat cells expressing CFL1. Blots were treated with (lanes 2, 3) or without (lane 1) lambda phosphatase for 1 hr at 37 °C. In lane 3, phosphatase treatments were performed in the presence of phospho-CFL1 (Ser-3) peptide. Blots were probed with CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900) (lane 1). Phosphatase treatment removes Ser3 phosphorylation of CFL1, but this phosphatase activity is reduced in the presence of peptide.Immunofluorescence
Immunocytochemical labeling in chick dorsal root ganglion neurons using CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900). (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CFL1
Entrez GeneID
1072Gene Name
CFL1
Gene Alias
CFL
Gene Description
cofilin 1 (non-muscle)
Omim ID
601442Gene Ontology
HyperlinkGene Summary
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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The ADF/cofilin family: actin-remodeling proteins.
Maciver SK, Hussey PJ.
Genome Biology 2002 Apr; 3(5):reviews300.
Application：WB, Human, Mammalian cells.
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Proteins of the ADF/cofilin family: essential regulators of actin dynamics.
Bamburg JR.
Annual Review of Cell and Developmental Biology 1999 Nov; 15:185.
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The ADF/cofilin family: actin-remodeling proteins.
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