Phosphotyrosine polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431, HeLa, and Jurkat cells (20 ug/lane) untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with Phosphotyrosine polyclonal antibody (Cat # PAB7897).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Phosphotyrosine.
Immunogen
peptide corresponding to a panel of phosphotyrosine-containing peptide of human protein.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunoprecipitation (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431, HeLa, and Jurkat cells (20 ug/lane) untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with Phosphotyrosine polyclonal antibody (Cat # PAB7897).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Antibodies for phosphotyrosine: analytical and preparative tool for tyrosyl-phosphorylated proteins.
Wang JY.
Analytical Biochemistry 1988 Jul; 172(1):1.
Application：AFC, IF, IP, WB-Ce, WB-Tr, Human, Mammalian cells, Recombinant proteins.
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Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten.
Ross AH, Baltimore D, Eisen HN.
Nature 1981 Dec; 294(5842):654.
Application：AFC, RIA, Mouse, Compounds, ANN-1 cells.
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Antibodies for phosphotyrosine: analytical and preparative tool for tyrosyl-phosphorylated proteins.
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