PLXNA1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blots showing mouse brain (lane 1), and COS-7 cells untransfected (lane 2) or transfected with mouse myc-tagged PLXNA1 (lane 3). The blots were probed with either affinity purified PLXNA1 polyclonal antibody (Cat # PAB7890).
Immunofluorescence
Immunocytochemical labeling using PLXNA1 polyclonal antibody (Cat # PAB7890) in COS-7 cells mock transfected (A) or transfected with Myc-tagged mouse PLXNA1 construct (B). The specificity of the binding in (B) was demonstrated by using PLXNA1 peptide in the presence of PLXNA1 polyclonal antibody (Cat # PAB7890) (C).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PLXNA1.
Immunogen
A synthetic peptide corresponding to the semaphorin domain in human PLXNA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:50)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blots showing mouse brain (lane 1), and COS-7 cells untransfected (lane 2) or transfected with mouse myc-tagged PLXNA1 (lane 3). The blots were probed with either affinity purified PLXNA1 polyclonal antibody (Cat # PAB7890).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling using PLXNA1 polyclonal antibody (Cat # PAB7890) in COS-7 cells mock transfected (A) or transfected with Myc-tagged mouse PLXNA1 construct (B). The specificity of the binding in (B) was demonstrated by using PLXNA1 peptide in the presence of PLXNA1 polyclonal antibody (Cat # PAB7890) (C).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLXNA1
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Interactome
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Pathway
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Disease
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Publication Reference
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The effect of cauda equina constriction on nitric oxide synthase activity.
Lukacova N, Kafka J, Cizkova D, Marsala M, Marsala J.
Neurochemical Research 2004 Feb; 29(2):429.
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Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates.
Tamagnone L, Artigiani S, Chen H, He Z, Ming GI, Song H, Chedotal A, Winberg ML, Goodman CS, Poo M, Tessier-Lavigne M, Comoglio PM.
Cell 1999 Oct; 99(1):71.
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The effect of cauda equina constriction on nitric oxide synthase activity.
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