WASF2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of rat PC-12 lysate. The blots were probed with WASF2 polyclonal antibody (Cat # PAB7889) in the presence (lane 2) or absence (lane 1) of WAVE2 (Central region) blocking peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WASF2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human WASF2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence has high homology with similar regions in rat and mouse WAVE2, and has low homology to similar regions in WAVE1 and WAVE3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of rat PC-12 lysate. The blots were probed with WASF2 polyclonal antibody (Cat # PAB7889) in the presence (lane 2) or absence (lane 1) of WAVE2 (Central region) blocking peptide.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WASF2
Entrez GeneID
10163Gene Name
WASF2
Gene Alias
SCAR2, WAVE2, dJ393P12.2
Gene Description
WAS protein family, member 2
Omim ID
605875Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. [provided by RefSeq
Other Designations
IMD2|OTTHUMP00000003471|WASP family Verprolin-homologous protein 2|suppressor of cyclic-AMP receptor (WASP-family)
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Interactome
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Pathway
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Publication Reference
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Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.
Leng Y, Zhang J, Badour K, Arpaia E, Freeman S, Cheung P, Siu M, Siminovitch K.
PNAS 2005 Jan; 102(4):1098.
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Crystal growth of carbonate apatite using a CaCO3 flux.
Suetsugu Y, Tanaka J.
Journal of Materials Science. Materials in Medicine 1999 Sep; 10(9):561.
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Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.
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