PLXNA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PLXNA1.
Immunogen
A synthetic peptide corresponding to N-terminus half of semaphorin domain in human PLXNA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:100)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blots showing neonatal rat brain. The blot was probed with either the affinity purified PLXNA1 polyclonal antibody (Cat # PAB7879).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling using PLXNA1 polyclonal antibody (Cat # PAB7879) in COS-7 cells mock transfected (A) or transfected with Myc-tagged mouse PLXNA1 construct (B). The specificity of the binding in (B) was demonstrated by using PLXNA1 peptide in the presence of PLXNA1 polyclonal antibody (Cat # PAB7879) (C).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLXNA1
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Interactome
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Pathway
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Disease
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Publication Reference
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Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes.
de Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW.
Journal of Extracellular Vesicles 2012 Apr; 1.
Application:WB-Ce, Human, HMEC-1 cells.
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The effect of cauda equina constriction on nitric oxide synthase activity.
Lukacova N, Kafka J, Cizkova D, Marsala M, Marsala J.
Neurochemical Research 2004 Feb; 29(2):429.
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Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates.
Tamagnone L, Artigiani S, Chen H, He Z, Ming GI, Song H, Chedotal A, Winberg ML, Goodman CS, Poo M, Tessier-Lavigne M, Comoglio PM.
Cell 1999 Oct; 99(1):71.
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Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes.
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