TUBB3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TUBB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TUBB3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is not found in bI or bII-tubulin isotypes, but is well conserved in bIII-tubulins from rat and mouse.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:200)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain. The blot was probed with TUBB3 polyclonal antibody (Cat # PAB7874).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling in chick dorsal root ganglion neurons using TUBB3 polyclonal antibody (Cat # PAB7874) . (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBB3
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Interactome
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Pathway
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Publication Reference
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Large-scale differentiation of iPSC-derived motor neurons from ALS and control subjects.
Michael J Workman, Ryan G Lim, Jie Wu, Aaron Frank, Loren Ornelas, Lindsay Panther, Erick Galvez, Daniel Perez, Imara Meepe, Susan Lei, Viviana Valencia, Emilda Gomez, Chunyan Liu, Ruby Moran, Louis Pinedo, Stanislav Tsitkov, Ritchie Ho, Julia A Kaye; Answer ALS Consortium; Terri Thompson, Jeffrey D Rothstein, Steven Finkbeiner, Ernest Fraenkel, Dhruv Sareen, Leslie M Thompson, Clive N Svendsen.
Neuron 2023 Apr; 111(8):1191.
Application:ICC, Quant, Human, iPS cells.
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
Fourest-Lieuvin A, Peris L, Gache V, Garcia-Saez I, Juillan-Binard C, Lantez V, Job D.
Molecular Biology of the Cell 2005 Dec; 17(3):1041.
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Post-translational modifications regulate microtubule function.
Westermann S, Weber K.
Nature Reviews. Molecular Cell Biology 2003 Dec; 4(12):938.
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Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fanarraga ML, Avila J, Zabala JC.
The European Journal of Neuroscience 1999 Feb; 11(2):517.
Application:WB, ELISA, Mouse, Mouse brain tubulin.
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Large-scale differentiation of iPSC-derived motor neurons from ALS and control subjects.
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