TUBB3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with TUBB3 polyclonal antibody (Cat # PAB7869).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TUBB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 168-177 of human TUBB3.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This sequence is identical to similar regions in bI, bII, and bIII-tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with TUBB3 polyclonal antibody (Cat # PAB7869).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBB3
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Interactome
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Pathway
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Publication Reference
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
Fourest-Lieuvin A, Peris L, Gache V, Garcia-Saez I, Juillan-Binard C, Lantez V, Job D.
Molecular Biology of the Cell 2005 Dec; 17(3):1041.
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Post-translational modifications regulate microtubule function.
Westermann S, Weber K.
Nature Reviews. Molecular Cell Biology 2003 Dec; 4(12):938.
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Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fanarraga ML, Avila J, Zabala JC.
The European Journal of Neuroscience 1999 Feb; 11(2):517.
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
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