PTK2 (phospho Y397) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extract from NIH/3T3 cells, using PTK2 (phospho Y397) polyclonal antibody (Cat # PAB7745).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using PTK2 (phospho Y397) polyclonal antibody (Cat # PAB7745).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y397 of human PTK2.
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from NIH/3T3 cells, using PTK2 (phospho Y397) polyclonal antibody (Cat # PAB7745).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using PTK2 (phospho Y397) polyclonal antibody (Cat # PAB7745). -
Gene Info — PTK2
Entrez GeneID
5747Protein Accession#
Q05397Gene Name
PTK2
Gene Alias
FADK, FAK, FAK1, pp125FAK
Gene Description
PTK2 protein tyrosine kinase 2
Omim ID
600758Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq
Other Designations
focal adhesion kinase 1
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Disease
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