NOS3 (phospho S1177) polyclonal antibody
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More Files
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Western Blot
Westeron Blot analysis of (1) extracts from 3T3 cells untreated, (2) treated with EGF, (3) HUVEC cells treated with VEGF, (4) JK cells untreated, and (5) JK cells treated with serum.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of NOS3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S1177 of human NOS3.
Host
Rabbit
Theoretical MW (kDa)
140
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Westeron Blot analysis of (1) extracts from 3T3 cells untreated, (2) treated with EGF, (3) HUVEC cells treated with VEGF, (4) JK cells untreated, and (5) JK cells treated with serum. -
Gene Info — NOS3
Entrez GeneID
4846Protein Accession#
P29474Gene Name
NOS3
Gene Alias
ECNOS, eNOS
Gene Description
nitric oxide synthase 3 (endothelial cell)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
endothelial nitric oxidase synthase|endothelial nitric oxide synthase
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