G3BP1 (phospho S232) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extract from 293 cells untreated or treated starvation using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719) (A) or preincubated with blocking peptide (B).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of G3BP1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S232 of human G3BP1.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from 293 cells untreated or treated starvation using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719) (A) or preincubated with blocking peptide (B).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using G3BP1 (phospho S232) polyclonal antibody (Cat # PAB7719). -
Gene Info — G3BP1
Entrez GeneID
10146Protein Accession#
Q13283Gene Name
G3BP1
Gene Alias
G3BP, HDH-VIII, MGC111040
Gene Description
GTPase activating protein (SH3 domain) binding protein 1
Omim ID
608431Gene Ontology
HyperlinkGene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
GAP binding protein|OTTHUMP00000160618|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
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Interactome
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