CARM1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells treated with EGF (200 ug/mL, 5 min) , using CARM1 polyclonal antibody (Cat # PAB7693).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 polyclonal antibody (Cat # PAB7693).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CARM1.
Immunogen
A synthetic peptide corresponding to residues surrounding S228 of human CARM1.
Host
Rabbit
Theoretical MW (kDa)
63
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells treated with EGF (200 ug/mL, 5 min) , using CARM1 polyclonal antibody (Cat # PAB7693).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 polyclonal antibody (Cat # PAB7693). -
Gene Info — CARM1
Entrez GeneID
10498Protein Accession#
A6NN38Gene Name
CARM1
Gene Alias
PRMT4
Gene Description
coactivator-associated arginine methyltransferase 1
Omim ID
603934Gene Ontology
HyperlinkGene Summary
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM
Other Designations
coactivator-associated arginine methyltransferase-1|protein arginine N-methyltransferase 4
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Interactome
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Disease
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