NPFFR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
NPFFR1 polyclonal antibody (Cat # PAB7586) (0.03 ug/mL) staining of human brain (cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NPFFR1.
Immunogen
A synthetic peptide corresponding to human NPFFR1.
Sequence
C-RPSGSHKEAYSERP
Host
Goat
Theoretical MW (kDa)
47.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NPFFR1 polyclonal antibody (Cat # PAB7586) (0.03 ug/mL) staining of human brain (cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NPFFR1
Entrez GeneID
64106Protein Accession#
NP_071429.1Gene Name
NPFFR1
Gene Alias
FLJ10751, GPR147, NPFF1, NPFF1R1, OT7T022
Gene Description
neuropeptide FF receptor 1
Omim ID
607448Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor 147|neuropeptide FF 1
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Pathway
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Disease
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Publication Reference
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Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding.
Gouarderes C, Mazarguil H, Mollereau C, Chartrel N, Leprince J, Vaudry H, Zajac JM.
Neuropharmacology 2007 Feb; 52(2):376.
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Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding.
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