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Last updated: 2016/12/4
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NOVA1 polyclonal antibody

  • Catalog # : PAB7585
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NOVA1.
  • Immunogen:
  • A synthetic peptide corresponding to human NOVA1.
  • Sequence:
  • C-REMPQNVAKTEPVS
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 51.7, 19.5
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:64000)
    Western blot (0.01-0.03 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NOVA1 polyclonal antibody (Cat # PAB7585) (0.01 ug/mL) staining of human breast cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • 293 overexpressing NOVA1 and probed with NOVA1 polyclonal antibody (Cat # PAB7585) (mock transfection in first lane), tested by Origene.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NOVA1 polyclonal antibody (Cat # PAB7585) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4857
  • Protein Accession#:
  • NP_002506.2;NP_006480.2;NP_006482.1
  • Gene Name:
  • NOVA1
  • Gene Alias:
  • Nova-1
  • Gene Description:
  • neuro-oncological ventral antigen 1
  • Gene Summary:
  • This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq
  • Other Designations:
  • paraneoplastic Ri antigen,ventral neuron-specific protein 1
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