NOVA1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NOVA1.
Immunogen
A synthetic peptide corresponding to human NOVA1.
Sequence
C-REMPQNVAKTEPVS
Host
Goat
Theoretical MW (kDa)
51.7, 19.5
Reactivity
Human
Specificity
This antibody is expected to recognize all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NOVA1 polyclonal antibody (Cat # PAB7585) (0.01 ug/mL) staining of human breast cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing NOVA1 and probed with NOVA1 polyclonal antibody (Cat # PAB7585) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NOVA1 polyclonal antibody (Cat # PAB7585) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOVA1
Entrez GeneID
4857Protein Accession#
NP_002506.2;NP_006480.2;NP_006482.1Gene Name
NOVA1
Gene Alias
Nova-1
Gene Description
neuro-oncological ventral antigen 1
Omim ID
602157Gene Ontology
HyperlinkGene Summary
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
paraneoplastic Ri antigen|ventral neuron-specific protein 1
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Interactome
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Disease
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Publication Reference
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Evolution of Nova-dependent splicing regulation in the brain.
Jelen N, Ule J, Zivin M, Darnell RB.
PLoS Genetics 2007 Oct; 3(10):1838.
Application:WB-Ti, Chicken, Mouse, Zebrafish, Brain, liver extract from, chicken, mouse, zebrafish.
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Evolution of Nova-dependent splicing regulation in the brain.
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