ILF2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ILF2 polyclonal antibody (Cat # PAB7568) (0.3 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ILF2 polyclonal antibody (Cat # PAB7568, 2.5 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence
ILF2 polyclonal antibody (Cat # PAB7568) (2.5 ug/mL) staining of parental and stable knock-down cell line (fixed by 4% formaldehyse, permealized by Triton X100). Primary incubation was 1.5 hour. Detected by fluorescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ILF2.
Immunogen
A synthetic peptide corresponding to human ILF2.
Sequence
C-EKAYEKPPEKKEG
Host
Goat
Theoretical MW (kDa)
43.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:16000)
Western blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
Immunofluorescence (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ILF2 polyclonal antibody (Cat # PAB7568) (0.3 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ILF2 polyclonal antibody (Cat # PAB7568, 2.5 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
ILF2 polyclonal antibody (Cat # PAB7568) (2.5 ug/mL) staining of parental and stable knock-down cell line (fixed by 4% formaldehyse, permealized by Triton X100). Primary incubation was 1.5 hour. Detected by fluorescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ILF2
Entrez GeneID
3608Protein Accession#
NP_004506.2Gene Name
ILF2
Gene Alias
MGC8391, NF45, PRO3063
Gene Description
interleukin enhancer binding factor 2, 45kDa
Omim ID
603181Gene Ontology
HyperlinkGene Summary
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq
Other Designations
interleukin enhancer binding factor 2|nuclear factor of activated T-cells, 45-kDa
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Interactome
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Publication Reference
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NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.
Sylvia Lai, Takuma Higuchi, Masayuki Tsuda, Yasunori Sugiyama, Keiko Morisawa, Taketoshi Taniguchi and Shuji Sakamoto.
Scientific Reports 2022 May; 12(1):8837.
Application：IHC-P, Mouse, mouse pancreas.
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A protein interaction network for pluripotency of embryonic stem cells.
Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH.
Nature 2006 Nov; 444(7117):364.
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NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.
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