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Last updated: 2016/12/4
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ILF2 polyclonal antibody

  • Catalog # : PAB7568
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ILF2.
  • Immunogen:
  • A synthetic peptide corresponding to human ILF2.
  • Sequence:
  • C-EKAYEKPPEKKEG
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 43.1
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:16000)
    Western blot (0.03-0.1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    Immunofluorescence (2-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ILF2 polyclonal antibody (Cat # PAB7568) (0.3 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ILF2 polyclonal antibody (Cat # PAB7568, 2.5 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • ILF2 polyclonal antibody (Cat # PAB7568) (2.5 ug/mL) staining of parental and stable knock-down cell line (fixed by 4% formaldehyse, permealized by Triton X100). Primary incubation was 1.5 hour. Detected by fluorescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3608
  • Gene Name:
  • ILF2
  • Gene Alias:
  • MGC8391,NF45,PRO3063
  • Gene Description:
  • interleukin enhancer binding factor 2, 45kDa
  • Gene Summary:
  • Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq
  • Other Designations:
  • interleukin enhancer binding factor 2,nuclear factor of activated T-cells, 45-kDa
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