ESRRG polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ESRRG.
Immunogen
A synthetic peptide corresponding to human ESRRG.
Sequence
CRMSNKDRHIDS
Host
Goat
Theoretical MW (kDa)
51.3, 48.6, 48.6
Reactivity
Human
Specificity
This antibody is expected to recognize the reported isoform NP_001429.2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.2-0.6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ESRRG polyclonal antibody (Cat # PAB7546) (0.2 ug/mL) staining of human breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing ESRRG and probed with ESRRG polyclonal antibody (Cat # PAB7546) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESRRG
Entrez GeneID
2104Protein Accession#
NP_001429.2;NP_996317.1;NP_996318.1Gene Name
ESRRG
Gene Alias
DKFZp781L1617, ERR3, FLJ16023, KIAA0832, NR3B3
Gene Description
estrogen-related receptor gamma
Omim ID
602969Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035076|OTTHUMP00000035092|OTTHUMP00000035095|OTTHUMP00000035115|OTTHUMP00000035116|OTTHUMP00000035126
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Interactome
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Disease
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Publication Reference
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Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma.
Dufour CR, Wilson BJ, Huss JM, Kelly DP, Alaynick WA, Downes M, Evans RM, Blanchette M, Giguere V.
Cell Metabolism 2007 May; 5(5):345.
Application:ChIP, IP, WB, Mouse, Mouse hearts.
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Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma.
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