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Last updated: 2016/12/4
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CRHR1 polyclonal antibody

  • Catalog # : PAB7539
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of CRHR1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 107-117 of human CRHR1.
  • Sequence:
  • C-NEEKKSKVHYH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 47.7
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • CRHR1 polyclonal antibody (Cat # PAB7539) (0.1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal image (3 ug/mL) of CRHR1 polyclonal antibody (Cat # PAB7539) (0.3 ug/mL) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1394
  • Gene Name:
  • CRHR1
  • Gene Alias:
  • CRF-R,CRF1,CRFR1,CRH-R1h,CRHR,CRHR1f
  • Gene Description:
  • corticotropin releasing hormone receptor 1
  • Gene Summary:
  • This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants
  • Other Designations:
  • CRF1 receptor isoform I variant B,corticotropin releasing hormone receptor variant 1h,corticotropin-releasing factor receptor,seven transmembrane helix receptor
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