CLIC4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CLIC4 polyclonal antibody (Cat # PAB7536) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence
CLIC4 polyclonal antibody (Cat # PAB7536) (5 ug/mL) staining of min6 cells transiently expressing mouse clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CLIC4.
Immunogen
A synthetic peptide corresponding to human CLIC4.
Sequence
NGLKEEDKEPLIE-C
Host
Goat
Theoretical MW (kDa)
28.8
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
Immunofluorescence (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CLIC4 polyclonal antibody (Cat # PAB7536) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunofluorescence
CLIC4 polyclonal antibody (Cat # PAB7536) (5 ug/mL) staining of min6 cells transiently expressing mouse clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLIC4
Entrez GeneID
25932Protein Accession#
NP_039234.1Gene Name
CLIC4
Gene Alias
CLIC4L, DKFZp566G223, FLJ38640, H1, MTCLIC, huH1, p64H1
Gene Description
chloride intracellular channel 4
Omim ID
606536Gene Ontology
HyperlinkGene Summary
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq
Other Designations
OTTHUMP00000003380|chloride intracellular channel 4 like
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Interactome
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Disease
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Publication Reference
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Proteomic profiling in Lipocalin 2 deficient mice under normal and inflammatory conditions.
Labbus K, Henning M, Borkham-Kamphorst E, Geisler C, Berger T, Mak TW, Knüchel R, Meyer HE, Weiskirchen R, Henkel C.
Journal of Proteomics 2013 Jan; 78:188.
Application：WB-Ti, Mouse, Liver.
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Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.
Suh KS, Crutchley JM, Koochek A, Ryscavage A, Bhat K, Tanaka T, Oshima A, Fitzgerald P, Yuspa SH.
Clinical Cancer Research 2000 Jun; 275(22):16986.
Application：IF, IHC, WB-Ce, Human, Human colon, esophagus, kidney from the tissue microarrays, Primary human dermal fibroblasts.
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Proteomic profiling in Lipocalin 2 deficient mice under normal and inflammatory conditions.
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