BHMT polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of BHMT.
Immunogen
A synthetic peptide corresponding to C-terminus of human BHMT.
Sequence
C-EQQLKELFEKQK
Host
Goat
Theoretical MW (kDa)
44.9
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
BHMT polyclonal antibody (Cat # PAB7525) (0.03 ug/mL) staining of rat liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing BHMT and probed with BHMT polyclonal antibody (Cat # PAB7525) (mock transfection in first lane), tested by Origene.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — BHMT
Entrez GeneID
635Protein Accession#
NP_001704.1Gene Name
BHMT
Gene Alias
-
Gene Description
betaine-homocysteine methyltransferase
Omim ID
602888Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq
Other Designations
betaine homocysteine methyltransferase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Regulation of FOXO3 by phosphorylation and methylation in hepatitis C virus infection and alcohol exposure.
Tikhanovich I, Kuravi S, Campbell RV, Kharbanda K, Artigues A, Villar MT, Weinman SA.
Hepatology 2014 Jan; 59(1):58.
-
Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice.
Collinsova M, Strakova J, Jiracek J, Garrow TA.
The Journal of Nutrition 2006 Jun; 136(6):1493.
Application:WB-Ti, Mouse, Liver.
-
Regulation of FOXO3 by phosphorylation and methylation in hepatitis C virus infection and alcohol exposure.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com