LDHC polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
LDHC polyclonal antibody (Cat # PAB7509) (0.03 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
LDHC polyclonal antibody (Cat # PAB7509) (1 ug/mL) staining of paraffin embedded mouse testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LDHC.
Immunogen
A synthetic peptide corresponding to amino acids 221-233 of human LDHC.
Sequence
C-DSDKEHWKNVHKQ
Host
Goat
Theoretical MW (kDa)
36.3
Reactivity
Mouse
Specificity
Both variants represent identical product (NP_002292.1 and NP_059144.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
LDHC polyclonal antibody (Cat # PAB7509) (0.03 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
LDHC polyclonal antibody (Cat # PAB7509) (1 ug/mL) staining of paraffin embedded mouse testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LDHC
Entrez GeneID
3948Protein Accession#
NP_002292.1;NP_059144.1Gene Name
LDHC
Gene Alias
CT32, LDH3, LDHX, MGC111073
Gene Description
lactate dehydrogenase C
Omim ID
150150Gene Ontology
HyperlinkGene Summary
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq
Other Designations
L-lactate dehydrogenase C|cancer/testis antigen 32
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Interactome
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Pathway
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Publication Reference
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Increased serum lactate dehydrogenase isoenzymes in Ph-negative chronic myeloproliferative diseases: a metabolic adaptation?
Mazzotta S, Guerranti R, Gozzetti A, Bucalossi A, Bocchia M, Sammassimo S, Petralia S, Ogueli GI, Lauria F.
Hematology 2006 Aug; 11(4):239.
Application：Func, Human, Serum.
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Increased serum lactate dehydrogenase isoenzymes in Ph-negative chronic myeloproliferative diseases: a metabolic adaptation?
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