EFHD2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EFHD2.
Immunogen
A synthetic peptide corresponding to human EFHD2.
Sequence
CAAFKELQSTFK
Host
Goat
Theoretical MW (kDa)
26.7
Reactivity
Human, Mouse
Specificity
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EFHD2 polyclonal antibody (Cat # PAB7501) (0.1 ug/mL) staining of human lymph node lysate. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EFHD2 polyclonal antibody (Cat # PAB7501) (0.3 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining. Positive cells A) in the interfollicular area and surrounding a vessel, B) in proximity of the epithelial criptae.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EFHD2
Entrez GeneID
79180Protein Accession#
NP_077305.2Gene Name
EFHD2
Gene Alias
MGC4342
Gene Description
EF-hand domain family, member D2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
EF hand domain containing 2|EF hand domain family, member D2|OTTHUMP00000002319|novel EF Hand domain-containing protein|swiprosin 1
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Interactome
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Publication Reference
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Identification of swiprosin 1 in human lymphocytes.
Vuadens F, Rufer N, Kress A, Corthesy P, Schneider P, Tissot JD.
Proteomics 2004 Aug; 4(8):2216.
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Identification of swiprosin 1 in human lymphocytes.
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