VPS35 polyclonal antibody (Cat # PAB7499) staining (1 ug/mL) of human cerebellum lysate (RIPA buffer, 35 ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
VPS35 polyclonal antibody (Cat # PAB7499) staining of paraffin embedded human testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3 ug/mL or B) Citrate buffer pH6 at 10ug/mL ., HRP-staining.
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq