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Last updated: 2017/12/10
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XBP1 polyclonal antibody

  • Catalog # : PAB7472
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of XBP1.
  • Immunogen:
  • A synthetic peptide corresponding to human XBP1.
  • Sequence:
  • C-NHSWEDTFANELFPQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 40.1, 28.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform XBP1(S) (NP_001073007.1) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • XBP1 polyclonal antibody (Cat # PAB7472) (1 ug/mL) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7494
  • Gene Name:
  • XBP1
  • Gene Alias:
  • TREB5,XBP2
  • Gene Description:
  • X-box binding protein 1
  • Gene Summary:
  • This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028682,X-box-binding protein-1
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