MYF5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MYF5.
Immunogen
A synthetic peptide corresponding to human MYF5.
Sequence
PECNSPVWSRKSST
Host
Goat
Theoretical MW (kDa)
28.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MYF5 polyclonal antibody (Cat # PAB7461) (0.5 ug/mL) staining of human muscle lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYF5
Entrez GeneID
4617Protein Accession#
NP_005584.1Gene Name
MYF5
Gene Alias
bHLHc2
Gene Description
myogenic factor 5
Omim ID
159990Gene Ontology
HyperlinkOther Designations
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Interactome
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Publication Reference
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Asymmetric self-renewal and commitment of satellite stem cells in muscle.
Kuang S, Kuroda K, Le Grand F, Rudnicki MA.
Cell 2007 Jun; 129(5):999.
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Asymmetric self-renewal and commitment of satellite stem cells in muscle.
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