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Last updated: 2017/7/16
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NRAS polyclonal antibody

  • Catalog # : PAB7450
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NRAS.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human NRAS.
  • Sequence:
  • C-RMKKLNSSDDGTQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 21.2
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (0.01-0.03 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NRAS polyclonal antibody (Cat # PAB7450) (0.1 ug/ml) staining of rat intestines lysate (35 ug protein in RIPA buffer).Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • NRAS polyclonal antibody (Cat # PAB7450) (0.01 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • 293 overexpressing NRAS and probed with NRAS polyclonal antibody (Cat # PAB7450) (mock transfection in first lane), tested by Origene.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NRAS polyclonal antibody (Cat # PAB7450) (2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4893
  • Gene Name:
  • NRAS
  • Gene Alias:
  • ALPS4,N-ras,NRAS1
  • Gene Description:
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • Gene Summary:
  • This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
  • Other Designations:
  • N-ras protein part 4,OTTHUMP00000013879,v-ras neuroblastoma RAS viral oncogene homolog
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