NRAS polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
NRAS polyclonal antibody (Cat # PAB7450) (0.1 ug/ml) staining of rat intestines lysate (35 ug protein in RIPA buffer).Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Cell lysate)
NRAS polyclonal antibody (Cat # PAB7450) (0.01 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing NRAS and probed with NRAS polyclonal antibody (Cat # PAB7450) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NRAS polyclonal antibody (Cat # PAB7450) (2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NRAS.
Immunogen
A synthetic peptide corresponding to internal region of human NRAS.
Sequence
C-RMKKLNSSDDGTQ
Host
Goat
Theoretical MW (kDa)
21.2
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NRAS polyclonal antibody (Cat # PAB7450) (0.1 ug/ml) staining of rat intestines lysate (35 ug protein in RIPA buffer).Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Cell lysate)
NRAS polyclonal antibody (Cat # PAB7450) (0.01 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing NRAS and probed with NRAS polyclonal antibody (Cat # PAB7450) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NRAS polyclonal antibody (Cat # PAB7450) (2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
NP_002515.1Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma.
Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K.
Cancer Epidemiology, Biomarkers & Prevention 2007 May; 16(5):991.
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Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma.
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