PC polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PC polyclonal antibody (Cat # PAB7443) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing Human PC and probed with PC polyclonal antibody (Cat # PAB7443) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PC.
Immunogen
A synthetic peptide corresponding to human PC.
Sequence
C-KFKEVKKAYVEANQ
Host
Goat
Theoretical MW (kDa)
130
Reactivity
Mouse
Specificity
All reported variants (NP_000911.2, NP_0010.35806.1, NP_071504.2) represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PC polyclonal antibody (Cat # PAB7443) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing Human PC and probed with PC polyclonal antibody (Cat # PAB7443) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PC
Entrez GeneID
5091Protein Accession#
NP_000911.2;NP_0010.35806.1;NP_071504.2Gene Name
PC
Gene Alias
PCB
Gene Description
pyruvate carboxylase
Gene Ontology
HyperlinkGene Summary
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme.
St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I.
Science 2007 Aug; 317(5841):1076.
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Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme.
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