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Last updated: 2022/6/26
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GOT1 polyclonal antibody 

  • Catalog # : PAB7415
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GOT1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 157-167 of human GOT1.
  • Sequence:
  • C-RSYRYWDAEKR
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 46.2
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000)
    Western blot (0.01-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • GOT1 polyclonal antibody (Cat # PAB7415) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GOT1 polyclonal antibody (Cat # PAB7415, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2805
  • Gene Name:
  • GOT1
  • Gene Alias:
  • GIG18
  • Gene Description:
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • Gene Summary:
  • Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020254,aspartate aminotransferase 1,growth-inhibiting protein 18
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