Goat polyclonal antibody raised against synthetic peptide of GOT1.
Immunogen:
A synthetic peptide corresponding to amino acids 157-167 of human GOT1.
Sequence:
C-RSYRYWDAEKR
Host:
Goat
Theoretical MW (kDa):
46.2
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:4000) Western blot (0.01-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
GOT1 polyclonal antibody (Cat # PAB7415) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations:
OTTHUMP00000020254,aspartate aminotransferase 1,growth-inhibiting protein 18