HADH polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
HADH polyclonal antibody (Cat # PAB7412) (0.02 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with HADH polyclonal antibody (Cat # PAB7412) in the left panel.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HADH.
Immunogen
A synthetic peptide corresponding to human HADH.
Sequence
C-YERGDASKEDID
Host
Goat
Theoretical MW (kDa)
34.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
HADH polyclonal antibody (Cat # PAB7412) (0.02 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with HADH polyclonal antibody (Cat # PAB7412) in the left panel.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HADH
Entrez GeneID
3033Protein Accession#
NP_005318.2Gene Name
HADH
Gene Alias
HAD, HADH1, HADHSC, HHF4, M/SCHAD, MGC8392, SCHAD
Gene Description
hydroxyacyl-Coenzyme A dehydrogenase
Gene Ontology
HyperlinkGene Summary
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq
Other Designations
L-3-hydroxyacyl-Coenzyme A dehydrogenase|L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Familial hyperinsulinemic hypoglycemia caused by a defect in the SCHAD enzyme of mitochondrial fatty acid oxidation.
Molven A, Matre GE, Duran M, Wanders RJ, Rishaug U, Njolstad PR, Jellum E, Sovik O.
Diabetes 2004 Jan; 53(1):221.
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Familial hyperinsulinemic hypoglycemia caused by a defect in the SCHAD enzyme of mitochondrial fatty acid oxidation.
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