PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of human adrenal gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Frozen sections)
PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30 min, Cy3-staining. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.