ASF1A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ASF1A polyclonal antibody (Cat # PAB7398) (1 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ASF1A.
Immunogen
A synthetic peptide corresponding to human ASF1A.
Sequence
C-ENSLNVMLESH
Host
Goat
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ASF1A polyclonal antibody (Cat # PAB7398) (1 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ASF1A
Entrez GeneID
25842Protein Accession#
NP_054753.1Gene Name
ASF1A
Gene Alias
CGI-98, CIA, DKFZp547E2110, HSPC146
Gene Description
ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Omim ID
609189Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq
Other Designations
ASF1 anti-silencing function 1 homolog A|CCG1-interacting factor A|anti-silencing function 1A
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Interactome
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Publication Reference
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Human Asf1 regulates the flow of S phase histones during replicational stress.
Groth A, Ray-Gallet D, Quivy JP, Lukas J, Bartek J, Almouzni G.
Molecular Cell 2005 Jan; 17(2):301.
Application：IP, Human, HeLa cells.
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Human Asf1 regulates the flow of S phase histones during replicational stress.
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