YWHAQ polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
YWHAQ polyclonal antibody (Cat # PAB7392) (0.03 ug/mL) staining of human brain (hippocampus) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of YWHAQ.
Immunogen
A synthetic peptide corresponding to human YWHAQ.
Sequence
C-DDRKQTIDNSQ
Host
Goat
Theoretical MW (kDa)
27.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
YWHAQ polyclonal antibody (Cat # PAB7392) (0.03 ug/mL) staining of human brain (hippocampus) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAQ
Entrez GeneID
10971Protein Accession#
NP_006817.1Gene Name
YWHAQ
Gene Alias
14-3-3, 1C5, HS1
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Omim ID
609009Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq
Other Designations
14-3-3 protein T-cell|14-3-3 protein tau|14-3-3 protein theta|OTTHUMP00000015730|protein tau|tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle.
Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M.
Diabetes 2007 Jun; 56(6):1608.
Application：WB-Ti, Human, Human skeletal muscle.
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Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle.
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