SETD2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SETD2 polyclonal antibody (Cat # PAB7385) (0.5 ug/mL) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SETD2.
Immunogen
A synthetic peptide corresponding to internal region of human SETD2.
Sequence
C-ERDPDKQTQNKE
Host
Goat
Theoretical MW (kDa)
231
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SETD2 polyclonal antibody (Cat # PAB7385) (0.5 ug/mL) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SETD2
Entrez GeneID
29072Protein Accession#
NP_054878.3Gene Name
SETD2
Gene Alias
FLJ16420, FLJ22472, FLJ23184, FLJ45883, FLJ46217, HIF-1, HSPC069, HYPB, KIAA1732, KMT3A, SET2, p231HBP
Gene Description
SET domain containing 2
Gene Ontology
HyperlinkGene Summary
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq
Other Designations
SET domain-containing protein 2|histone-lysine N-methyltransferase SETD2|huntingtin interacting protein 1|huntingtin yeast partner B|huntingtin-interacting protein B|lysine N-methyltransferase 3A
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Interactome
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Pathway
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Disease
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Publication Reference
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SETD2 non genomic loss of function in advanced systemic mastocytosis is mediated by an Aurora kinase A/MDM2 axis and can be therapeutically targeted.
Manuela Mancini, Cecilia Monaldi, Sara De Santis, Cristina Papayannidis, Michela Rondoni, Chiara Sartor, Samantha Bruno, Livio Pagano, Marianna Criscuolo, Roberta Zanotti, Massimiliano Bonifacio, Patrizia Tosi, Michel Arock, Peter Valent, Michele Cavo, Simona Soverini.
Biomarker Research 2023 Mar; 11(1):29.
Application：Co-IP, WB-Ce, Human, Bone marrow mononuclear, HMC-1, ROSA cells.
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SETD2 and histone H3 lysine 36 methylation deficiency in advanced systemic mastocytosis.
Martinelli G, Mancini M, De Benedittis C, Rondoni M, Papayannidis C, Manfrini M, Meggendorfer M, Calogero R, Guadagnuolo V, Fontana MC, Bavaro L, Padella A, Zago E, Pagano L, Zanotti R, Scaffidi L, Specchia G, Albano F, Merante S, Elena C, Savini P, Gangemi D, Tosi P, Ciceri F, Poletti G, Riccioni L, Morigi F, Delledonne M, Haferlach T, Cavo M, Valent P, Soverini S.
Leukemia 2017 Jun; [Epub].
Application：IP-WB, WB-Ce, WB-Ti, Human, HMC-1.1, HMC-1.2, K-562 cells, Human systemic mastocytosis tissues, Mononuclear cells of healthy donors.
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Identification and characterization of a novel human histone H3 lysine 36-specific methyltransferase.
Sun XJ, Wei J, Wu XY, Hu M, Wang L, Wang HH, Zhang QH, Chen SJ, Huang QH, Chen Z.
The Journal of Biological Chemistry 2005 Oct; 280(42):35261.
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SETD2 non genomic loss of function in advanced systemic mastocytosis is mediated by an Aurora kinase A/MDM2 axis and can be therapeutically targeted.
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